WebApr 12, 2024 · The relationship between sleep and Parkinson’s is complex, but irrespective of cause and/or effect, the circadian clock can explain many of the underlying mechanisms of the disease. ... How decreases in BMAL1 levels and a disrupted circadian cycle may lead to Parkinson’s is still unclear. We know that reducing BMAL1 expression in mice ... WebWe tested the nonredundant clock gene Bmal1 in intestinal homeostasis and tumorigenesis, using the Apc min model of colorectal cancer. Methods: Bmal1 mutant, epithelium-conditional Bmal1 mutant, and photoperiod (day/night cycle) disrupted mice bearing the Apc min allele were assessed for tumorigenesis. Tumors and normal …
The role of clock genes in sleep, stress and memory
WebBy impairing the EC-specific function of the circadian clock transcriptional activator BMAL1 in vivo, we detect angiogenesis defects in mouse neonatal vascular tissues, as well as in adult tumor angiogenic settings. We then investigate the function of circadian clock machinery in cultured EC and show evidence that BMAL and CLOCK knock-down ... WebSep 30, 2008 · Bmal1-dependent genes Dbp and Rev-erbα exhibited a nearly complete loss of expression in the liver but showed normal rhythmic expression in skeletal muscle, and Cry1, a core clock gene, showed the up-regulated expression expected in the absence of Bmal1 function only in the liver (Fig. 2D). cellular theory
BMAL1 coordinates energy metabolism and differentiation of pluripotent ...
We then explored how depletion of BMAL1 affects end-resection. As chromatin remodeling regulated by histone modification, in particular acetylation of Histone H4 (H4Ac), is critical for DNA end-resection in eukaryotes and HR-mediated DSBs repair [12, 42, 43]. CLOCK has an intrinsic acetyltransferase domain … See more To find out whether BMAL1 directly participates in DSBR, human sarcoma U2OS cells were treated with Zeocin, a radio-mimetic chemical that predominantly induces DSBs [22, … See more DNA end-resection is the key event that controls the DSB repair pathway choice, which generates the long ssDNA leading cells prone to HR-mediated repair [37]. Because BMAL1 … See more We then explored how depletion of BMAL1 affects DSBR. BMAL1 was knocked down in U2OS, the amount of DSBs was determined by detecting DNA fragments released by DSBs and the level of phosphorylation of … See more It is well recognized that cancer cells that are deficient in HR-mediated DSBR are frequently more sensitive to killing by chemotherapy drugs such as etoposide or irradiation than the … See more WebSep 22, 2024 · CLOCK:BMAL1 was among the top enriched DNA binding motifs, confirming that the core clock is needed for rhythmic expression of these genes (fig. S4B). In addition, there was significant enrichment for several known liver metabolic regulators, such as HNF4α, CEBPB, and FOXA (fig. S4B). To investigate whether activities of these TFs … WebApr 13, 2024 · PcDNA and siRNA were used to upregulate and downregulate Bmal1 and confirmed that Bmal1 inhibited LPS-triggered ferroptosis in H9c2 cells. Then, the results suggested that AKT/p53 pathway was restrained by LPS in H9c2 cell. ... We performed RT-qPCR to test a panel of circadian gene mRNA, including Bmal1, Clock, Per1, Per2, … cellular therapeutics cdmo