Web29 dec. 2005 · Alpha-ketoglutarate dehydrogenase (alpha-KGDH) is a highly regulated enzyme, which could determine the metabolic flux through the Krebs cycle. It catalyses … Web30 okt. 2010 · In Saccharomyces cerevisiae, KGDH is a mitochondrial macromolecular multienzyme complex consisting of three components: alpha-ketoglutarate …
RCSB PDB - 5TRM: Crystal structure of human GCN5 histone ...
Web20 mrt. 2024 · By analyzing the co-crystal structures of the catalytic domain of KAT2A in complex with acetyl-CoA, propionyl-CoA, butyryl-CoA, malonyl-CoA, ... Liu R, Xia Y, Tan L, Yang P, Lee JH, Li XJ, et al. KAT2A coupled with the alpha-KGDH complex acts as a histone H3 succinyltransferase. Nature. 2024;552(7684):273–277. Web11 mei 2024 · Dihydrolipoyl dehydrogenase is a component of the alpha-KGDH complex, which is considered to be a major source for the production of ROS [36–38]. Therefore, we next examined the effect of SNO on the ROS production of the alpha-KGDH complex using an in vitro assay with purified enzyme. msu vs michigan prediction
Generation of Reactive Oxygen Species in the Reaction Catalyzed …
Web5 jan. 2024 · Although succinyl-CoA can be converted from α-KG by the α-KGDH complex, which is composed of oxoglutarate dehydrogenase (OGDH), dihydrolipoyl succinyltransferase (DLST), and dihydrolipoyl dehydrogenase (DLD), in the mitochondria, α-KGDH is also distributed in the nucleus in an NLS of DLST-dependent manner in … Web26 okt. 2024 · Metabolic enzymes can form complexes with other proteins and regulate the functions of these proteins via their metabolite products. Our research group and others have demonstrated that nucleus-localized acetyl-CoA synthetase short-chain family member 2 (ACSS2), ACLY, PDC, α-KGDH, and fumarase are in complex with chromatin … Cells were collected and washed twice with ice-cold PBS, then resuspended in triton extraction buffer (TEB: PBS containing 0.5% Triton X-100 (v/v), 2 mM phenylmethylsulfonyl fluoride (PMSF), 0.02% (w/v) NaN3) at a cell density of 107 cells per ml. Cells were then lysed on ice for 10 min with gentle … Meer weergeven The antibodies used in the experiments are listed in Supplementary Table 9. DNase-free RNase A and propidium iodide were … Meer weergeven Parental U87, U251, and 293 cells were obtained from the American Type Culture Collection. Parental U87 and U251 cell lines used in the experiments were authenticated … Meer weergeven To identify KAT2A-dependent succinylation of lysines on histone H3, an in vitro KAT2A-succinylated sample of purified histone H3 was exhaustively acetylated … Meer weergeven PCR-amplified human OGDH, DLST, and DLD were cloned into pcDNA6V5HisB and pcDNA3.1/hygro(+)-Flag vectors. A full-length KAT2A … Meer weergeven how to make money in january