Topo pcr ii dual promotor thermofisher
WebSep 4, 2024 · RT-PCR products were either sequenced directly or cloned into the pCRII-TOPO vector with dual promoter (ThermoFisher Scientific) for sequencing. The 5’terminus of the virus was determined using the SMARTer® RACE 5'/3' Kit (Takara Bio USA, Inc., Madison. WI) with the genome-specific primer LN529 ( Table 1 ). WebThey use a pCR™-TOPO™ Vector with covalently bound topoisomerase I for fast cloning and recombinants. pCR™-TOPO™ Vectors include: • 3´-T overhangs for direct ligation of … TOPO™ TA Cloning™ Kits are designed for cloning PCR products directly from a PCR … TaqMan Real-Time PCR Assays. Antibodies. Oligos, Primers & Probes
Topo pcr ii dual promotor thermofisher
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WebThese kits include the pCR™II-TOPO™ TA vector with dual T7 and SP6 promoters. By eliminating time-consuming and tedious restriction site cloning, TOPO™ cloning is the … WebThey use a pCR™-TOPO™ Vector with covalently bound topoisomerase I for fast cloning and recombinants. pCR™-TOPO™ Vectors include: • 3´-T overhangs for direct ligation of …
WebThermo Fisher pcrii topo dual promoter cloning vector Pcrii Topo Dual Promoter Cloning Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, … WebIncludes the pCR™II-TOPO TA vector with dual T7 and SP6 promoters. By eliminating time-consuming and tedious restriction site cloning, TOPO cloning is the most reliable cloning method, featuring a 5-minute cloning reaction and 3 …
WebPcrii Topo Dual Promoter Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more Average 86 stars, based on 1 Price from $9.99 to $1999.99 WebIntroduction The HTP TOPO TA Cloning® Dual Promoter Kits are specifically designed to simplify high-throughput (HTP) applications. Sufficient pCR ®II-TOPO is provided in bulk …
WebDual Promoter (pCR®II ) Catalog Numbers K2050-01, K2050-40, K2060-01, K2060-40, K2070-20, and K2070-40 . Document Part Number 250176 Publication Number …
WebTOPO TA Cloning Dual Promoter kits are for fast, efficient cloning and subsequent in vitro transcription. These kits include the pCRII-TOPO TA vector with dual T7 and SP6 promoters. By eliminating time-consuming and tedious rest tlr 8x usedWebTOPO TA Cloning® Kit Dual Promoter pCR® II-TOPO Vector. Restriction Enzyme Analysis. ... Thermo Fisher Scientific. Life Sciences Solutions. 5781 Van Allen Way. Carlsbad, CA, USA 92008. www.thermofisher.com For inquiries, contact us at . [email protected]. Anita Targosz. QA Associate Director. tlr 8ight e 3.0WebDesigned for cloning PCR products directly from a PCR reaction in just 5 minutes Shop Invitrogen™ TOPO™ TA Cloning™ Kit, Dual Promoter, with One Shot™ Invitrogen™ TOPO™ TA Cloning™ Kit, Dual Promoter, with One Shot™ TOP 10F' Chemically Competent E.coli, no manual 25 reactions products Fisher Scientific tlr 8a flexWebThey use a pCR-TOPO Vector with covalently bound topoisomerase I for fast cloning and recombinants. pCR-TOPO TOPO™ TA Cloning™ Kit, Dual Promoter, with pCR™II-TOPO™ Vector and One Shot™ TOP10 Electrocomp™ E. coli tlr agencyWebFast—5-minute, room temperature reaction; Simple—add restriction sites and/or universal primer sites to either end of your PCR product in just 3 easy steps; Efficient—up to 95% of … tlr 8a streamlightWebInvitrogen™ TOPO™ TA Cloning™ Kit, Dual Promoter, with pCR™II-TOPO™ Vector and One Shot™ TOP10F' Chemically Competent E. coli Designed for cloning PCR products directly from a PCR reaction in just 5 minutes 754.00 EUR - … tlr 8ight xt setup sheetWebAsp718 I Ecl136 II Dra II Comments for pCR®-Blunt II-TOPO® 3519 nucleotides lac promoter/operator region: bases 95-216 M13 Reverse priming site: bases 205-221 LacZ-alpha ORF: bases 217-576 SP6 promoter priming site: bases 239-256 Multiple Cloning Site: bases 269-399 TOPO®-Cloning site: bases 336-337 T7 promoter priming site: bases 406 … tlr adaptor family